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Kjemisk dimeriseringsindusert proteinkondensater på telomerer
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Kjemisk dimeriseringsindusert proteinkondensater på telomerer

Article DOI: 10.3791/62173-v • 08:52 min • April 12th, 2021
April 12th, 2021 •
1, 2, 1
1Department of Biological Sciences, Mellon College of Science, Carnegie Mellon University, 2Department of Chemistry, School of Arts and Sciences, University of Pennsylvania

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Denne protokollen illustrerer et kjemisk indusert protein dimeriseringssystem for å skape kondensater på kromatin.  Dannelsen av promyelocytisk leukemi (PML) kjernelegeme på telomerer med kjemiske dimerizere er demonstrert. Dråpevekst, oppløsning, lokalisering og sammensetning overvåkes med levende celleavbildning, immunfluorescens (IF) og fluorescens in situ hybridisering (FISH).

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Biologi utgave 170 kondensater væske-væske fase separasjon kjemisk dimmerizer lokal kondensasjon telomerer PML kjernefysisk organ
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