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Journal / Immunology and Infection / 监测人类痰样中的中性粒细胞和导管素 G…
JoVE Journal
Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
监测人类痰样中的中性粒细胞和导管素 G 活性
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监测人类痰样中的中性粒细胞和导管素 G 活性

Article DOI: 10.3791/62193-v • 09:23 min • May 21st, 2021
May 21st, 2021 •
*1,2, *1,2,3,4, 1,2,5,6,7, 1,3,8
1Translational Lung Research Center Heidelberg (TLRC), German Center for Lung Research (DZL), 2Dept. of Translational Pulmonology, University of Heidelberg, 3Molecular Medicine Partnership Unit (MMPU), European Molecular Biology Laboratory (EMBL), University of Heidelberg, 4Faculty of Biosciences, Collaboration for Joint Ph.D. Degree between EMBL and Heidelberg University, University of Heidelberg, 5Dept. of Pediatric Pulmonology, Immunology and Critical Care Medicine, Charité – Universitätsmedizin Berlin, 6Berlin Institute of Health (BIH), 7German Center for Lung Research (DZL), Associated Partner Site, Berlin, 8Dept. of Chemical Physiology and Biochemistry, Oregon Health and Science University
* These authors contributed equally

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Summary
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Click here for the English version.

本文所述的协议提供了一个指南，可视化和量化人类痰中中性粒细胞蛋白酶的活动。这种分析的应用范围从抗炎治疗的评估，到生物标志物验证、药物筛选和大队列临床研究。

Tags

免疫学和感染， 第 171 期， 囊性纤维化， 肺病， 炎症， 蛋白酶， FRET 记者， 中性粒细胞酶， 导管素 G， 诊断
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