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Developmental Biology

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JoVE Journal Developmental Biology
Erzeugung und Expansion menschlicher Kardiomyozyten aus mononukleären Zellen des patientenperipheren Blutes
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Journal (Developmental Biology)
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Erzeugung und Expansion menschlicher Kardiomyozyten aus mononukleären Zellen des patientenperipheren Blutes

Article DOI: 10.3791/62206-v • 05:38 min • February 12th, 2021
February 12th, 2021 •
1,2, 3, 1,2, 1,2,4, 1,2,5, 3, 1,2,5,6
1Center for Cardiovascular Research, The Abigail Wexner Research Institute, Nationwide Children’s Hospital, 2The Heart Center, Nationwide Children’s Hospital, 3Department of Physiology and Cell Biology, The Ohio State University College of Medicine, 4Department of Anatomy, The Ohio State University College of Medicine, 5MCDB Graduate Program, The Ohio State University, 6Department of Pediatrics, The Ohio State University College of Medicine

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Hier stellen wir ein Protokoll zur robusten Erzeugung und Erweiterung menschlicher Kardiomyozyten aus mononukleären Zellen des patienteneigenen peripheren Blutes vor.

Tags

Entwicklungsbiologie Ausgabe 168 periphere mononukleäre Blutzellen PBMCs humane induzierte pluripotente Stammzellen iPS-Zellen kardiale Differenzierung iPSC-Reprogrammierung Kardiomyozytenexpansion.
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