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Journal / Biology / Leverans av Cas9/sgRNA Ribonucleoprotein Complex…
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Leverans av Cas9/sgRNA Ribonucleoprotein Complex i odödliga och primära celler via virusliknande partiklar ("Nanoblades")
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Leverans av Cas9/sgRNA Ribonucleoprotein Complex i odödliga och primära celler via virusliknande partiklar ("Nanoblades")

Article DOI: 10.3791/62245-v • 09:23 min • March 31st, 2021
March 31st, 2021 •
2, 1, 1, 1
1LBMC, Laboratoire de Biologie et Modélisation de la Cellule Univ Lyon, ENS de Lyon, Université Claude Bernard Lyon 1, CNRS, UMR 5239, INSERM, U1210, Lyon, 69007, France, 2CIRI, Centre International de Recherche en Infectiologie Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, F-69007, Lyon, France

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Vi har utvecklat ett enkelt och billigt protokoll för att ladda Cas9/single-guide RNA (sgRNA) ribonukleoproteinkomplex inom virusliknande partiklar. Dessa partiklar, som kallas "Nanoblades", möjliggör effektiv leverans av Cas9/sgRNA-komplexet i odödliga och primära celler samt in vivo.

Tags

Biologi Nummer 169 CRISPR Cas9 ribonucleoprotein RNP Murine Leukemia Virus Virusliknande partiklar VLP virusvektor proteinleverans
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