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JoVE Journal
Developmental Biology

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使用rx3:GFP转基因斑马鱼通过光片显微镜可视化眼部形态发生
 
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使用rx3:GFP转基因斑马鱼通过光片显微镜可视化眼部形态发生

Article DOI: 10.3791/62296-v 07:40 min April 5th, 2021
April 5th, 2021

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Summary

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Click here for the English version.

在这里,提供了一种方案,用于使用市售的光照相显微镜和图像处理工作站对眼部形态发生进行延时成像,以分析结果数据。该协议详细说明了胚胎麻醉的程序,嵌入低熔点温度琼脂糖,悬浮在成像室中,设置成像参数,最后使用图像分析软件分析成像数据。

Tags

发育生物学,第170期,延时成像,光照相图,实时成像,斑马鱼,眼睛发育,视网膜
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