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Journal / Biology / Kultur metoder til at studere apikale-specifikke…
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Biology

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JoVE Journal Biology
Kultur metoder til at studere apikale-specifikke interaktioner ved hjælp af tarmorganoid modeller
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Kultur metoder til at studere apikale-specifikke interaktioner ved hjælp af tarmorganoid modeller

Article DOI: 10.3791/62330-v • 07:49 min • March 23rd, 2021
March 23rd, 2021 •
*1, *2, *2, 1, 2, 2,3, 1, 2
1STEMCELL Technologies Ltd., 2STEMCELL Technologies Inc., 3Terry Fox Laboratory, BC Cancer
* These authors contributed equally

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Her præsenterer vi to protokoller, der gør det muligt at modellere tarmapikaspecifikke interaktioner. Organoid-afledte tarmmonolag og AIR-Liquid Interface (ALI) kulturer letter generering af veldifferentierede epithelia tilgængelige fra både lysarms- og basolaterale sider, mens polaritetsindvendte tarmorganoider udsætter deres apikale side og kan opnås til høje gennemløbsanalyser.

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Biologi tarmorganoid apicobasal polaritet tarmmonomerer cellekulturmembranindsatser polaritetsinversion modeller
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