Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Forberedelse og kryo-FIB mikromaskiner af…
JoVE Journal
Biochemistry
Author Produced

This content is Free Access.

JoVE Journal Biochemistry
Forberedelse og kryo-FIB mikromaskiner af Saccharomyces cerevisiae til Kryo-Elektron Tomografi
Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
Journal (Biochemistry)
Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum
Journal (Biochemistry)
Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum
 
Click here for the English version

Forberedelse og kryo-FIB mikromaskiner af Saccharomyces cerevisiae til Kryo-Elektron Tomografi

Article DOI: 10.3791/62351-v • 09:06 min • November 20th, 2021
November 20th, 2021 •
1, 1, 1, 1
1Central European Institute of Technology, Masaryk University

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Vi præsenterer en protokol for lameller forberedelse af springet frosne biologiske prøver af kryo-fokuserede ion stråle mikromaskiner til høj opløsning strukturelle undersøgelser af makromolekyler in situ med cryo-elektron tomografi. Den fremlagte protokol indeholder retningslinjer for udarbejdelse af lameller af høj kvalitet med høj reproducerbarhed til strukturel karakterisering af makromolekyler inde i Saccharomyces cerevisiae.

Tags

Biokemi udgave 177
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter