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Journal / Neuroscience / Tab af funktionsmetode i den embryonale chick…
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JoVE Journal Neuroscience
Tab af funktionsmetode i den embryonale chick nethinden ved hjælp af Tol2 transposon-medieret transgen ekspression af kunstige mikroRNA'er
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Tab af funktionsmetode i den embryonale chick nethinden ved hjælp af Tol2 transposon-medieret transgen ekspression af kunstige mikroRNA'er

Article DOI: 10.3791/62399-v • 06:58 min • May 18th, 2022
May 18th, 2022 •
1, 1
1Department of Biology, Valparaiso University

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Vi har udviklet en ny tab-af-funktion tilgang, der involverer introduktion og genomisk integration af kunstige mikro-RNA-sekvenser i kyllingembryoner ved hjælp af i ovo-elektroporation og Tol2-transposonsystemet. Denne teknik giver en robust og stabil genknockdown-metode til undersøgelser af genfunktion under udvikling.

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Retraktion udgave 183 i ovo-elektroporation kyllingembryo nethinden Tol2-transposon miRNA genmålretning
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