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Journal / Biochemistry / 基于质谱的蛋白质组学方法用于全局和高置信度的蛋白质R-甲基化分析…
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Biochemistry

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JoVE Journal Biochemistry
基于质谱的蛋白质组学方法用于全局和高置信度的蛋白质R-甲基化分析
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基于质谱的蛋白质组学方法用于全局和高置信度的蛋白质R-甲基化分析

Article DOI: 10.3791/62409-v • 09:40 min • April 28th, 2022
April 28th, 2022 •
*1,2, *1, 1,2, 1
1Department of Experimental Oncology, European Institute of Oncology IRCCS (IEO), 2European School of Molecular Medicine (SEMM), c/o Campus IFOM-IEO
* These authors contributed equally

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Summary
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蛋白精氨酸（R）-甲基化是一种广泛的翻译后修饰，调节多种生物学途径。质谱法是全球分析R-甲基蛋白质组的最佳技术，当与修饰肽富集的生化方法相结合时。本文介绍了专为高置信度鉴定人细胞中全球R-甲基化而设计的工作流程。

Tags

生物化学，第 182 期，R-甲基化，蛋白质组学，质谱，蛋白质精氨酸甲基转移酶，hmSILAC，免疫亲和富集，HpH-RP 色谱分级分离
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