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Journal / Biology / In vitro Hastalık Modellemesi için İnsan Primer…
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In vitro Hastalık Modellemesi için İnsan Primer Kapak Hücrelerinin İzolasyonu
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In vitro Hastalık Modellemesi için İnsan Primer Kapak Hücrelerinin İzolasyonu

Article DOI: 10.3791/62439-v • 07:31 min • April 16th, 2021
April 16th, 2021 •
1, 1, 1, 1, 2, 1,3
1Division of Cardiology, Department of Medicine, and the Pittsburgh Heart, Lung, and Blood Vascular Medicine Institute, University of Pittsburgh, 2Division of Cardiac Surgery, Department of Cardiothoracic Surgery, University of Pittsburgh and Heart and Vascular Institute, University of Pittsburgh Medical Center, 3Department of Bioengineering, University of Pittsburgh

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Bu protokol, cerrahi aort kapak replasman prosedürleri sırasında veya kadavra dokusundan ekstrakte edilen insan aort kapaklarının toplanmasını ve ardından hastaya özgü primer kapak endotel ve interstisyel hücrelerinin izolasyonunu, genişlemesini ve karakterizasyonunu tanımlar. Hücre canlılığını ve fenotip spesifikliğini sağlamak için gereken süreçlerle ilgili önemli detaylar dahildir.

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