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Journal / Immunology and Infection / Påvisning af SARS-CoV-2 neutraliserende…
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Immunology and Infection

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JoVE Journal Immunology and Infection
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Påvisning af SARS-CoV-2 neutraliserende antistoffer ved hjælp af fluorescerende billeddannelse af pseudovirusinfektion

Article DOI: 10.3791/62486-v • 10:25 min • June 5th, 2021
June 5th, 2021 •
1,2, 1,2, 1,2, 1, 1,2, 1,2, 1,2
1Ottawa Hospital Research Institute, 2Department of Biochemistry, Microbiology and Immunology, University of Ottawa

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Den protokol, der er beskrevet her skitserer en hurtig og effektiv metode til måling neutraliserende antistoffer mod SARS-CoV-2 spike protein ved at evaluere evnen af rekonvalescerende serum prøver til at hæmme infektion med en forbedret grøn fluorescerende protein-mærket vesikulær stomatitis virus pseudotypeeret med spike glycoprotein.

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Immunologi og infektion Udgave 172 SARS-CoV-2 COVID-19 Pseudovirus Neutraliserende antistof Vesikulær stomatitisvirus Spike glycoprotein
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