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Journal / Biochemistry / Proteomic Profil af EPS-Urin gennem FASP…
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Biochemistry
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JoVE Journal Biochemistry
Proteomic Profil af EPS-Urin gennem FASP Fordøjelse og data-uafhængig analyse
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Proteomic Profil af EPS-Urin gennem FASP Fordøjelse og data-uafhængig analyse

Article DOI: 10.3791/62512-v • 14:48 min • May 8th, 2021
May 8th, 2021 •
1, 1, 2, 3, 3, 1, 4, 1
1Research Centre for Advanced Biochemistry and Molecular Biology, Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro, 2Department of Health Science, Magna Graecia University of Catanzaro, 3Romolo Hospital, 4Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro

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Her præsenterer vi en optimeret in-filter fordøjelsesprotokol med detaljerede oplysninger om følgende: protein fordøjelse, peptidrensning og data uafhængig erhvervelse analyse. Denne strategi anvendes til analyse af udtrykte prostatiske sekreter-urinprøver og giver mulighed for høj proteom dækning og lav manglende værdi etiket-fri profilering af urin proteome.

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