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Journal / Biochemistry / Enkel in-house ultra-højtydende kapillær kolonne…
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Biochemistry
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JoVE Journal Biochemistry
Enkel in-house ultra-højtydende kapillær kolonne fremstilling med FlashPack-tilgangen
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Enkel in-house ultra-højtydende kapillær kolonne fremstilling med FlashPack-tilgangen

Article DOI: 10.3791/62522-v • 13:36 min • December 4th, 2021
December 4th, 2021 •
1, 1, 2
1Laboratory of Bioinformatics Methods in Combinatorial Chemistry and Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 2Department of Molecular Neuroimmune Signalling, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

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Her præsenterer vi en protokol til den optimerede FlashPack kapillær kolonne pakning procedure. Anvendelse af en optimeret protokol til en fælles 100-bar trykbombe setup giver mulighed for 10 gange hurtigere pakning og fremstilling af lange ultra-højtydende kapillær kolonner.

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Biokemi Udgave 178 kapillærkromatografi søjlepakning proteomics flydende kromatografi
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