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Journal / Biochemistry / RIBO-seq i bakterier: en prøvesamling og…
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JoVE Journal Biochemistry
RIBO-seq i bakterier
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RIBO-seq i bakterier: en prøvesamling og bibliotekforberedelsesprotokoll for NGS-sekvensering

Article DOI: 10.3791/62544-v • 12:05 min • August 7th, 2021
August 7th, 2021 •
1,2, 1,2, 1,3, 1,2, 1,2
1Laboratory of Gene Expression, ECOTECH-Complex, Maria Curie-Sklodowska University, 2Department of Molecular Biology, Institute of Biological Sciences, Maria Curie-Sklodowska University, 3Faculty of Information Technology, Polish-Japanese Academy of Information Technology

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Her beskriver vi stadier av prøvetaking og forberedelse for RIBO-seq i bakterier. Sekvensering av bibliotekene utarbeidet i henhold til disse retningslinjene resulterer i tilstrekkelige data for omfattende bioinformatisk analyse. Protokollen vi presenterer er enkel, bruker standard laboratorieutstyr og tar syv dager fra lysis til å skaffe biblioteker.

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Biokjemi utgave 174
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