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JoVE Journal
Immunology and Infection

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使用 1-Cyano-4-二甲基亚胺四氟化物 (CDAP) 激活和结合可溶性聚糖
 
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使用 1-Cyano-4-二甲基亚胺四氟化物 (CDAP) 激活和结合可溶性聚糖

Article DOI: 10.3791/62597-v 07:20 min June 14th, 2021
June 14th, 2021

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蛋白质和含胺配体可与氰化试剂、1-氰化物-4-二甲基甲基苯丙胺四氟溴酸酯(CDAP)激活的多糖共价相关,形成共价蛋白(利甘)-多糖结合体。本文描述了在 0 °C 和变 pH 值下执行受控 CDAP 激活以及执行激活多糖后续结合的改进协议。

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免疫学和感染,第172期,
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