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Immunology and Infection

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JoVE Journal Immunology and Infection
Longitudinal Follow-Up of Urinary Tract Infections and Their Treatment in Mice using Bioluminescence Imaging
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Longitudinal Follow-Up of Urinary Tract Infections and Their Treatment in Mice using Bioluminescence Imaging

Article DOI: 10.3791/62614-v • 07:39 min • June 14th, 2021
June 14th, 2021 •
1, 2, 1, 1, 1, 3, 1, 4
1Laboratory of Ion Channel Research (LICR), VIB-KU Leuven Center for Brain & Disease Research, Leuven, Belgium & Department of Cellular and Molecular Medicine, KU Leuven, 2Biomedical MRI, Department of Imaging & Pathology, KU Leuven, 3Belgium & KU Leuven Centre of Microbial and Plant Genetics, VIB-KU Leuven Center for Microbiology, Leuven, 4Laboratory of Experimental Urology, Department of Development and Regeneration, KU Leuven

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Summary
Automatic Translation

This manuscript describes the intravesical administration of uropathogenic bacteria with a lux operon to induce a urinary tract infection in mice and subsequent longitudinal in vivo analysis of the bacterial load using bioluminescence imaging.

Tags

Longitudinal Follow-up Urinary Tract Infections Treatment Mice Bioluminescence Imaging Colony Forming Units Reproducibility UTI Field In Vivo Research Bladder Physiology UTI Pathogenesis Susceptibility Real-time Insight Evolution Of Infection Animals Needed Recurrent Infections Chronic Infections Ascending Infections Dissemination To Blood Glycerol Stock Of Bacteria LB Plates Kanamycin Sulfate Culturing Overnight Polystyrene Round Bottom Tube Snap Caps LB Broth Inoculation Loop
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