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In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter
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In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter

Article DOI: 10.3791/62643-v • 05:04 min • February 22nd, 2022
February 22nd, 2022 •
*1,2, *1, 1
1Department of Cardiac Surgery, Cardiovascular Center, The University of Michigan, Ann Arbor, 2Department of Cardiovascular Medicine, Xiangya Hospital, Central South University
* These authors contributed equally

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Summary
Automatic Translation

We describe here, the establishment and application of an Tg(Myh6-cre)1Jmk/J /Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/J (referred to as αMHC-Cre/Rosa26A-Flox-Stop-Flox-GCaMP3 below) mouse reporter line for cardiac reprogramming assessment. Neonatal cardiac fibroblasts (NCFs) isolated from the mouse strain are converted into induced cardiomyocytes (iCMs), allowing for convenient and efficient evaluation of reprogramming efficiency and functional maturation of iCMs via calcium (Ca2+) flux.

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In Vitro Assessment Cardiac Reprogramming Calcium Flux GCaMP3 Reporter Functional Maturation ICMs Evaluation Procedures Reproducibility Cardiomyocyte Differentiation Cardiac Function Neonatal Cardiac Fibroblasts Fluorescence Microscope Genotype Isolation Of Hearts DPBS Blood Cell Pollution Trypsin-EDTA
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