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In vitro vurdering af hjerte omprogrammering ved måling af hjertespecifik calciumflux med en GCaMP3 Reporter
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In vitro vurdering af hjerte omprogrammering ved måling af hjertespecifik calciumflux med en GCaMP3 Reporter

Article DOI: 10.3791/62643-v • 05:04 min • February 22nd, 2022
February 22nd, 2022 •
*1,2, *1, 1
1Department of Cardiac Surgery, Cardiovascular Center, The University of Michigan, Ann Arbor, 2Department of Cardiovascular Medicine, Xiangya Hospital, Central South University
* These authors contributed equally

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Vi beskriver her etableringen og anvendelsen af en Tg(Myh6-cre)1Jmk/J /Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/J (benævnt αMHC-Cre/Rosa26A-Flox-Stop-Flox-GCaMP3 nedenfor) musereporterlinje til omprogrammering af hjerte. Neonatale hjertefibroblaster (NCF'er) isoleret fra musestammen omdannes til inducerede kardiomyocytter (iCMs), hvilket giver mulighed for bekvem og effektiv evaluering af omprogrammeringseffektivitet og funktionel modning af iCMs via calcium (Ca2+) flux.

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