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Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint
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Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

Article DOI: 10.3791/62718-v • 06:06 min • July 22nd, 2021
July 22nd, 2021 •
1, 1, 2, 1,3
1Bone and Joint Center, Henry Ford Health System, 2Department of Orthopedic Surgery, Henry Ford Health System, 3Center for Molecular Medicine and Genetics, Wayne State University

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Summary
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Primary tissues obtained from patients following total knee arthroplasty provide an experimental model for osteoarthritis research with maximal clinical translatability. This protocol describes how to identify, process, and isolate RNA from seven unique knee tissues to support mechanistic investigation in human osteoarthritis.

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Tissue Collection RNA Extraction Human Osteoarthritic Knee Joint Gene Expression Analysis Histological Analysis Primary Human Tissues Clinical Model Mechanistic Insights Identification Of Knee Joint Components High Quality RNA Extraction Downstream Gene Expression Assays Diseased Joint Tissues Clean And Cool Environment Hard Tissues Soft Tissues Specimen Sterile PBS Rinse Two Millimeter Cubes Cryo Vial Flash Freezing
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