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Journal / Biology / Mesoscopic Optical Imaging of Whole Mouse Heart
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JoVE Journal Biology
Mesoscopic Optical Imaging of Whole Mouse Heart
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Mesoscopic Optical Imaging of Whole Mouse Heart

Article DOI: 10.3791/62795-v • 08:53 min • October 14th, 2021
October 14th, 2021 •
*1, *1, *1, 1, 1,2, 1,3,4, 1,5, 1,3,4, 1,3,6
1European Laboratory for Non-Linear Spectroscopy, 2Department of Biology, University of Florence, 3National Institute of Optics, National Research Council, 4Department of Physics and Astronomy, University of Florence, 5Department of Neurosciences, Psychology, Drugs and Child Health, University of Florence, 6Institute for Experimental Cardiovascular Medicine, Faculty of Medicine, University of Freiburg
* These authors contributed equally

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Summary
Automatic Translation

We report a method for mesoscopic reconstruction of the whole mouse heart by combining new advancements in tissue transformation and staining with the development of an axially scanned light-sheet microscope.

Tags

Mesoscopic Optical Imaging Whole Mouse Heart Clearing Protocol Optical Sectioning Mesos PIM Technology Micrometric Resolution Cardiac Tissues Mouse Hearts Single Scan Three-dimensional Tissue Organization Cannulated Heart Tyro Solution 4% Paraform Aldehyde 0.01 Molar PBS Wash Heart Sodium Azide Hydrogel Solution De-gas Sample Dryer Vacuum Pump Nitrogen Pipeline
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