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Bioengineering

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JoVE Journal Bioengineering
Automatiseret mikrobiel dyrkning og adaptiv evolution ved hjælp af mikrobielt mikrodrøblekultursystem (MMC)
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Automatiseret mikrobiel dyrkning og adaptiv evolution ved hjælp af mikrobielt mikrodrøblekultursystem (MMC)

Article DOI: 10.3791/62800-v • 08:18 min • February 18th, 2022
February 18th, 2022 •
*1,2, *1,2,3, 4, 1,2,6, 1,2,5, 3, 1,2,5
1Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, 2Key Laboratory of Industrial Biocatalysis, Ministry of Education, Tsinghua University, 3Luoyang TMAXTREE Biotechnology Co., Ltd., 4Biochemical Engineering Research Group, School of Chemical Engineering and Technology, Xi’an Jiaotong University, 5Center for Synthetic & Systems Biology, Tsinghua University, 6School of Life Science and Technology, Tokyo Institute of Technology
* These authors contributed equally

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Denne protokol beskriver, hvordan man bruger det mikrobielle mikrodropletkultursystem (MMC) til at udføre automatiseret mikrobiel dyrkning og adaptiv evolution. MMC kan dyrke og underdyrke mikroorganismer automatisk og kontinuerligt og overvåge deres vækst online med relativt høj gennemstrømning og god parallelisering, hvilket reducerer arbejdskraft- og reagensforbruget.

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Bioengineering udgave 180 mikrobielt mikrodrømmekultursystem automatiserede operationer høj gennemstrømning mikrobiel dyrkning adaptiv laboratorieudvikling onlinedetektion
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