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Bildereplikatoriske domener i ultrastrukturelt bevart kromatin av elektrontomografi
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Bildereplikatoriske domener i ultrastrukturelt bevart kromatin av elektrontomografi

Article DOI: 10.3791/62803-v • 14:56 min • May 20th, 2022
May 20th, 2022 •
*1,2, *1,2, *1,3, 1,2, 1, 1, 1, 2, 4, 1,2,5
1Belozersky Institute of Physico-chemical Biology, Lomonosov Moscow State University, 2Faculty of Biology, Lomonosov Moscow State University, 3Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 4National Research Center, Kurchatov Institute, 5V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology
* These authors contributed equally

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Denne protokollen presenterer en teknikk for høyoppløselig kartlegging av replikeringssteder i strukturelt bevart kromatin in situ som bruker en kombinasjon av pre-embedding EdU-streptavidin-Nanogold merking og ChromEMT.

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Biologi utgave 183
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