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JoVE Journal Cancer Research
In Vivo Immunogenicitet Screening av tumör-härledda extracellulära vesicles av flöde cytometri av splenic T-celler
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In Vivo Immunogenicitet Screening av tumör-härledda extracellulära vesicles av flöde cytometri av splenic T-celler

Article DOI: 10.3791/62811-v • 08:02 min • September 23rd, 2021
September 23rd, 2021 •
1,2, 1,2,3,4, 1,2
1Department of Medicine III, School of Medicine, Technical University of Munich, 2Center for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of Munich, 3Department of Internal Medicine III, University Hospital Regensburg, 4National Centre for Tumor Diseases WERA

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Detta manuskript beskriver hur man bedömer in vivo immunogenicity av tumör cell-härledda extracellulära blåsor (EVs) med hjälp av flöde cytometri. EVs härrör från tumörer som genomgår behandling-inducerad immunogenic cell död verkar särskilt relevant i tumör immunosurveillance. Detta protokoll exemplifierar bedömningen av oxaliplatin-inducerad immunostimulatory tumör EVs men kan anpassas till olika inställningar.

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Cancerforskning nummer 175
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