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Neuroscience

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JoVE Journal Neuroscience
Generación de Unidades Motoras Humanas con Uniones Neuromusculares Funcionales en Dispositivos Microfluídicos
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Generación de Unidades Motoras Humanas con Uniones Neuromusculares Funcionales en Dispositivos Microfluídicos

Article DOI: 10.3791/62959-v • 10:48 min • September 7th, 2021
September 7th, 2021 •
1,2, 1,2, 1,2, 1,2, 3,4, 3,4, 5, 3,4, 1,2, 1,2, 5, 1,2,6, 1,2
1Department of Neurosciences, Experimental Neurology, and Leuven Brain Institute, KU Leuven - University of Leuven, 2VIB Center for Brain & Disease Research, Laboratory of Neurobiology, , 3VIB Center for Brain & Disease Research, Research Group Molecular Neurobiology, , 4VIB Bio Imaging Core, KU Leuven - University of Leuven, 5Department of Development and Regeneration, Stem Cell and Developmental Biology, KU Leuven - University of Leuven, 6Department of Neurology, University Hospitals Leuven

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Describimos un método para generar unidades motoras humanas en dispositivos microfluídicos disponibles comercialmente mediante el cocultivo de neuronas motoras derivadas de células madre pluripotentes inducidas por humanos con miotubos derivados de mesoangioblastos primarios humanos, lo que resulta en la formación de uniones neuromusculares funcionalmente activas.

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Neurociencia Número 175
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