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Journal / Bioengineering / İnsan Primer Cilt Fibroblastlarına Dayalı…
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
İnsan Primer Cilt Fibroblastlarına Dayalı Huntington Hastalık Modelinde Mikrotübül Artı-Uç Dinamikleri Görselleştirmesi
Imaging Integrin Tension and Cellular Force at Submicron Resolution with an Integrative Tension Sensor
Journal (Bioengineering)
Imaging Integrin Tension and Cellular Force at Submicron Resolution with an Integrative Tension Sensor
Probing the Roles of Physical Forces in Early Chick Embryonic Morphogenesis
Journal (Bioengineering)
Probing the Roles of Physical Forces in Early Chick Embryonic Morphogenesis
 
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İnsan Primer Cilt Fibroblastlarına Dayalı Huntington Hastalık Modelinde Mikrotübül Artı-Uç Dinamikleri Görselleştirmesi

Article DOI: 10.3791/62963-v • 10:38 min • January 8th, 2022
January 8th, 2022 •
*1,2, *2,3, 1,2, 3,4, 3,4, 1,3
1A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 3Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 4Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
* These authors contributed equally

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Bu protokol, birincil hücre kültüründeki dinamik özelliklerini incelemek için EB3 protein transfeksiyonu ile mikrotübül artı-uç görselleştirmesine adanmıştır. Protokol, Huntington hastalığı hastalarından elde edilen insan primer deri fibroblastları üzerinde uygulandı.

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Biyomühendislik Sayı 179 mikrotübüller transfeksiyon EB3 birincil kültür Huntington hastalığı yaşam boyu mikroskopi
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