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Neuroscience

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JoVE Journal Neuroscience
Aislamiento magnético de células microgliales de ratones neonatos para cultivos celulares primarios
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Aislamiento magnético de células microgliales de ratones neonatos para cultivos celulares primarios

Article DOI: 10.3791/62964-v • 07:23 min • July 25th, 2022
July 25th, 2022 •
*1, *1,2, 1, 1, 1, 1, 1, 1, 1,2, 1, 1
1NeuroDiderot, Inserm UMR-1141, Hôpital Robert Debré 48, Université de Paris, 2Department of anesthesia and critical care, APHP-Sorbonne university
* These authors contributed equally

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Los cultivos primarios de microglía se utilizan comúnmente para evaluar nuevas moléculas antiinflamatorias. El presente protocolo describe un método reproducible y relevante para aislar magnéticamente la microglía de cachorros neonatos.

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Neurociencia Número 185 Microglía cultivo celular neuroinflamación ensayo fagocítico expresión génica aislamiento magnético
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