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JoVE Journal Environment
En standardiseret procedure til overvågning af skadelige algeopblomstringer i Chile ved metabarcodinganalyse
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En standardiseret procedure til overvågning af skadelige algeopblomstringer i Chile ved metabarcodinganalyse

Article DOI: 10.3791/62967-v • 09:47 min • August 26th, 2021
August 26th, 2021 •
1, 1, 2, 3, 3, 3, 4, 5, 6, 6, 4, 4, 7, 5, 3, 1
1Office of Research and Academia-Government-Community Collaboration, Hiroshima University, 2Graduate School of Human and Environmental Studies, Kyoto University, 3Scientific and Biotechnological Bioresource Nucleus, Universidad de La Frontera, 4Centro de Estudios de Algas Nocivas, Instituto de Fomento Pesquero, 5Ciencias del Mar y Recursos Biologicos, Universidad de Antofagasta, 6Departamento de Ciencias Biológicas y Biodiversidad, Universidad de Los Lagos, 7Japan Fisheries Research and Education Agency, Fisheries Resources Institute

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Denne protokol introducerer trin til metabarcoding analyse, rettet mod 16S rRNA og 18S rRNA gener, til overvågning af skadelige algeopblomstringer og deres tilhørende mikrobiom i havvand prøver. Det er et kraftfuldt molekylært baseret værktøj, men kræver flere procedurer, som visuelt forklares her trin for trin.

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Miljøvidenskab Udgave 174 skadelige algeopblomstringer Chile HAB overvågning metabarkodning analyse algebakteriel mutualisme fytoplankton laks akvakultur
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