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Immunology and Infection

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JoVE Journal Immunology and Infection
Flowcytometrisk analyse til identifikation af de medfødte og adaptive immunceller i Murine Lung
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Flowcytometrisk analyse til identifikation af de medfødte og adaptive immunceller i Murine Lung

Article DOI: 10.3791/62985-v • 09:57 min • November 16th, 2021
November 16th, 2021 •
1,2, 1,2,3, 1,2, 1,2, 1,2
1Division of Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 2Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 3Harvard College

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I denne undersøgelse præsenterer vi en effektiv og reproducerbar protokol til at isolere immunpopulationerne i murine åndedrætssystemet. Vi leverer også en metode til identifikation af alle medfødte og adaptive immunceller, der befinder sig i lungerne hos raske mus, ved hjælp af et 9-farvebaseret flowcytometripanel.

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Immunologi og infektion udgave 177 lunge immunceller gating strategi flowcytometri
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