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Journal / Bioengineering / 合成生物学と天然物アプリケーションのための高収量スト レプトマイス…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
合成生物学と天然物アプリケーションのための高収量スト レプトマイス 転写翻訳ツールキット
Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications
Journal (Bioengineering)
Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications
Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
Journal (Bioengineering)
Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
 
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合成生物学と天然物アプリケーションのための高収量スト レプトマイス 転写翻訳ツールキット

Article DOI: 10.3791/63012-v • 07:59 min • September 10th, 2021
September 10th, 2021 •
1,2,3,4, 5, 5, 1,2,3,4,6,7, 5
1Centre for Synthetic Biology and Innovation, South Kensington Campus, 2Department of Medicine, South Kensington Campus, 3Section of Structural and Synthetic Biology, Department of Infectious Disease, Imperial College London, 4Sir Alexander Fleming Building, South Kensington Campus, 5School of Biosciences, Division of Natural Sciences, University of Kent, 6UK Dementia Research Institute Care Research and Technology Centre, Imperial College London; Hammersmith Campus, 7UK Innovation and Knowledge Centre for Synthetic Biology (SynbiCITE) and the London Biofoundry, Imperial College Translation & Innovation Hub

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このプロトコルは、 ストレプトマイセスベネズエラ 細胞非転写変換(TX-TL)システムから組み換えタンパク質の高収率を合成するための強化された方法を詳述する。

Tags

バイオエンジニアリング、第175号、無細胞タンパク質合成、 インビトロ 転写翻訳、 ストレプトマイセス、合成生物学、システム生物学、無細胞システム、生合成
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