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Indsamling, udvidelse og differentiering af primære humane nasale epitelcellemodeller til kvantificering af cilia beatfrekvens
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Indsamling, udvidelse og differentiering af primære humane nasale epitelcellemodeller til kvantificering af cilia beatfrekvens

Article DOI: 10.3791/63090-v • 11:13 min • November 10th, 2021
November 10th, 2021 •
1,2, 1,2, 1,2,3, 1,2, 1,2,3, 4, 5, 1,2,3
1School of Women's and Children's Health, Faculty of Medicine and Health, University of New South Wales, 2Molecular and Integrative Cystic Fibrosis Research Centre (miCF RC), Faculty of Medicine and Health, University of New South Wales, 3Department of Respiratory Medicine, Sydney Children's Hospital, 4Department of Pathology, School of Medical Sciences, University of New South Wales Australia, 5Katharina Gaus Light Microscopy Facility, Mark Wainwright Analytical Centre, University of New South Wales

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Denne protokol beskriver nasal epitelcelleindsamling, ekspansion og differentiering til organotypiske luftvejsepitelcellemodeller og kvantificering af cilia-beatfrekvens via levende cellebilleddannelse og specialbyggede scripts.

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Medicin udgave 177
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