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Journal / Neuroscience / İntraserebral Transplantasyon ve Fare Beynindeki…
JoVE Journal
Neuroscience

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JoVE Journal Neuroscience
İntraserebral Transplantasyon ve Fare Beynindeki İnsan Nöral Progenitör Hücrelerinin İnVivo Biyolüminesans İzlenmesi
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İntraserebral Transplantasyon ve Fare Beynindeki İnsan Nöral Progenitör Hücrelerinin İnVivo Biyolüminesans İzlenmesi

Article DOI: 10.3791/63102-v • 06:12 min • January 27th, 2022
January 27th, 2022 •
1,2, 1, 1, 1, 1, 1, 1, *1,2, *1,2
1Institute for Regenerative Medicine, University of Zurich, 2Neuroscience Center Zurich, University of Zurich and ETH Zurich
* These authors contributed equally

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Fare beyninde lusiferaz-yeşil floresan proteini (GFP) eksprese eden ikili muhabir bir vektör ile transdüke edilen insan nöral progenitör hücrelerinin intraparankimal transplantasyonunu tanımladık. Transplantasyondan sonra, lusiferaz sinyali, floresan mikroskobu kullanılarak beyin bölümlerinde tanımlanan in vivo biyolüminesans ve GFP eksprese eden aşılanmış hücreler kullanılarak tekrar tekrar ölçülür.

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Nörobilim Sayı 179 Hücre transplantasyonu lusiferaz in vivo görüntüleme GFP beyin kesiti intraparankimal transplantasyon
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