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Et trippelkulturcellesystem som modellerer den menneskelige blod-hjernebarrieren
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Et trippelkulturcellesystem som modellerer den menneskelige blod-hjernebarrieren

Article DOI: 10.3791/63134-v • 09:21 min • November 30th, 2021
November 30th, 2021 •
*1, *1, 1, 2, 3, 3, 3, 2, 1, 1, 1
1Univ. Artois, UR 2465, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), 2Queen Mary University of London, Department of Chemistry, 3Yamaguchi University Graduate School of Medicine, Department of Neurology and Clinical Neuroscience
* These authors contributed equally

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Denne protokollen beskriver en metode for å etablere en human blod-hjernebarriere (BBB) in vitro-modell . Endotelceller og pericytter blir sådd på hver side av et innsatsfilter (blodrom), og astrocytter blir sådd i bunnbrønnen (hjernekammeret). Den karakteriserte modellen ble brukt til nanopartikler transporteksperimenter.

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