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Imaging confocale ad alto rendimento di trimeri spike SARS-CoV-2 coniugati quantum dot per tracciare il legame e l'endocitosi nelle cellule HEK293T
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Imaging confocale ad alto rendimento di trimeri spike SARS-CoV-2 coniugati quantum dot per tracciare il legame e l'endocitosi nelle cellule HEK293T

Article DOI: 10.3791/63202-v • 06:39 min • April 21st, 2022
April 21st, 2022 •
1, 2, 2,3, 2, 1
1National Center for Advancing Translational Sciences, 2Optical Sciences Division, Code 5600, Naval Research Laboratory, 3Jacobs Corporation

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In questo protocollo, i punti quantici coniugati al picco RICOMBINANTE SARS-CoV-2 consentono saggi basati su cellule per monitorare il legame dello spike a hACE2 sulla membrana plasmatica e la successiva endocitosi delle proteine legate nel citoplasma.

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Biologia Numero 182
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