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JoVE Journal Neuroscience
Lasermikrodissektionsbaseret protokol til LC-MS/MS-analyse af den proteomiske profil af neuromelaningranulat
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Lasermikrodissektionsbaseret protokol til LC-MS/MS-analyse af den proteomiske profil af neuromelaningranulat

Article DOI: 10.3791/63289-v • 07:35 min • December 16th, 2021
December 16th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2, 3,4, 5, 1,2, *1,2, *1,2
1Medizinisches Proteom-Center, Medical Faculty, Ruhr-University Bochum, 2Medical Proteome Analysis, Center for Proteindiagnostics (PRODI), Ruhr-University Bochum, 3Center of Mental Health; Clinic and Policlinic for Psychiatry, Psychosomatics and Psychotherapy, University Hospital Wuerzburg, 4Psychiatry Department of Clinical Research, University of Southern Denmark Odense University Hospital, 5Center of Mental Health, Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, University Hospital of Wuerzburg, University of Wuerzburg
* These authors contributed equally

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En robust protokol præsenteres her til isolering af neuromelaningranulat fra humant post mortem substantia nigra pars compacta-væv via lasermikrodissektion. Denne reviderede og optimerede protokol minimerer massivt den krævede tid til prøveindsamling, reducerer den krævede prøvemængde og forbedrer identifikationen og kvantificeringen af proteiner ved LC-MS / MS-analyse.

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Neurovidenskab udgave 178 Neuromelaningranulat lasermikrodissektion massespektrometri substantia nigra pars compacta parallel reaktionsovervågning dataafhængig erhvervelse
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