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JoVE Journal Neuroscience
Måleegenskaper for det periodiske skjelettet i axon-segmentet ved hjelp av 3D-strukturert belysningsmikroskopi (3D-SIM)
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Måleegenskaper for det periodiske skjelettet i axon-segmentet ved hjelp av 3D-strukturert belysningsmikroskopi (3D-SIM)

Article DOI: 10.3791/63327-v • 07:40 min • February 11th, 2022
February 11th, 2022 •
1, 2, 1,3,4
1Minerva Foundation Institute for Medical Research, 2Department of Computer Science, Aalto University School of Science, 3HiLIFE - Neuroscience Center, University of Helsinki, 4HiLIFE - Institute of Biotechnology, University of Helsinki

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Den nåværende protokollen beskriver en metode for å visualisere og måle aktinringer og andre komponenter i membran periodisk skjelett av axon innledende segmentet ved hjelp av dyrkede rotte hippocampal nevroner og 3D-strukturert belysning mikroskopi (3D-SIM).

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