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Journal / Developmental Biology / Isolierung von mesenchymalen Stammzellen des…
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JoVE Journal Developmental Biology
Isolierung von mesenchymalen Stammzellen des Fettgewebes von Ratten zur Differenzierung in insulinproduzierende Zellen
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Isolierung von mesenchymalen Stammzellen des Fettgewebes von Ratten zur Differenzierung in insulinproduzierende Zellen

Article DOI: 10.3791/63348-v • 08:09 min • August 29th, 2022
August 29th, 2022 •
1, 2, 2, 1,2,3
1Biochemistry Department, Faculty of Pharmacy, Ain Shams University, 2Pharmacology and Biochemistry Department, Faculty of Pharmacy, The British University in Egypt, 3Center for Drug Research and Development (CDRD), Faculty of Pharmacy, The British University in Egypt

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Aus Fettgewebe gewonnene mesenchymale Stammzellen (Ad-MSCs) können eine potenzielle Quelle für MSCs sein, die sich in insulinproduzierende Zellen (IPCs) differenzieren. In diesem Protokoll stellen wir detaillierte Schritte zur Isolierung und Charakterisierung von epididymalen Ad-MSCs von Ratten bereit, gefolgt von einem einfachen, kurzen Protokoll zur Generierung von IPCs aus denselben Ratten-Ad-MSCs.

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Entwicklungsbiologie Ausgabe 186 Stammzellen mesenchymale Stammzellen Diabetes insulinproduzierende Zellen Fettgewebe Differenzierung
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