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Journal / Neuroscience / Måling af iltforbrugshastighed i akutte striatale …
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JoVE Journal Neuroscience
Måling af iltforbrugshastighed i akutte striatale skiver fra voksne mus
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Måling af iltforbrugshastighed i akutte striatale skiver fra voksne mus

Article DOI: 10.3791/63379-v • 07:41 min • June 8th, 2022
June 8th, 2022 •
1, 1, 1, 1, 1,2, 1, 3, 4, 1
1Department of Neuroscience, Thomas Jefferson University, 2School of Biomedical Engineering, Drexel University, 3Department of Pathology, MitoCare Center, Thomas Jefferson University, 4School of Life Sciences, Peking University

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Oxygenforbrugshastighed (OCR) er en fælles proxy for mitokondriefunktion og kan bruges til at studere forskellige sygdomsmodeller. Vi udviklede en ny metode ved hjælp af en Seahorse XF-analysator til direkte måling af OCR i akutte striatale skiver fra voksne mus, der er mere fysiologisk relevant end andre metoder.

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Neurovidenskab Udgave 184 Iltforbrugshastighed OCR mitokondrie respiration mus akut hjerneskive XF24 analysator striatum Parkinsons sygdom
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