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JoVE Journal Neuroscience
En tyngdekraftsfodret transkardieperfusionsmetode til histologisk analyse af musens centralnervesystem
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En tyngdekraftsfodret transkardieperfusionsmetode til histologisk analyse af musens centralnervesystem

Article DOI: 10.3791/63386-v • 10:37 min • January 21st, 2022
January 21st, 2022 •
1, 2, 1,3
1Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, 2Department of Neurology, State University of New York Upstate Medical University, 3Department of Neuroscience and Physiology, State University of New York Upstate Medical University

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En bekvem tyngdekraftsfodret perfusionsmetode til histologisk analyse af musens centralnervesystem præsenteres. Immunofluorescerende påvisning af phosphoryleret α-synuclein er demonstreret i en musemodel af Parkinsons sygdom. Dette arbejde beskriver også omfattende transkardieperfusion, dissektion, vævsfrysning / indlejring og frosne sektioneringstrin.

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Neurovidenskab udgave 179
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