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JoVE Journal Bioengineering
Engineering antivirale middelen via Surface Plasmon Resonance
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Engineering antivirale middelen via Surface Plasmon Resonance

Article DOI: 10.3791/63541-v • 13:00 min • June 14th, 2022
June 14th, 2022 •
1,2
1Department of Environmental Health Sciences, University of California, Los Angeles, 2Department of General Surgery, Medical University of Vienna

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Het huidige protocol beschrijft nieuwe hulpmiddelen voor SPR-bindingstests om CV-N-binding aan HA, S-glycoproteïne, gerelateerde hybride-type glycanen en oligosacchariden met een hoog mannosegehalte te onderzoeken. SPR wordt gebruikt om de KD te bepalen voor het binden van dimerische of monomere CV-N aan deze glycanen.

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Bio-engineering Cyanovirin-N oppervlakte plasmon resonantie verzadiging transfer verschil-NMR envelope spikes glycaan recombinant glycoproteïne
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