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Svært multiplekset vevsavbildning med Raman Fargestoffer

Article DOI: 10.3791/63547-v • 07:18 min • April 21st, 2022
April 21st, 2022 •
1, 1, 1,2
1Department of Chemistry, Columbia University, 2Kavli Institute for Brain Science, Columbia University

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Elektronisk pre-resonans stimulert Raman spredning (epr-SRS) avbildning av regnbue-lignende Raman fargestoffer er en ny plattform for svært multiplekset epitop-basert protein avbildning. Her presenterer vi en praktisk guide, inkludert antistoffpreparat, vevsprøvefarging, SRS mikroskopmontering og epr-SRS vevsavbildning.

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Bioengineering utgave 182 stimulert Raman spredningsmikroskopi elektronisk pre-resonans svært multiplekset proteinavbildning Raman fargestoffer immunhiistokjemi
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