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JoVE Journal Neuroscience
Confocale microscopie om drie modi van fusieporiëndynamiek in bijnierchromaffinecellen te meten
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Confocale microscopie om drie modi van fusieporiëndynamiek in bijnierchromaffinecellen te meten

Article DOI: 10.3791/63569-v • 12:30 min • March 16th, 2022
March 16th, 2022 •
*1, *1, 1, 1
1National Institute of Neurological Disorders and Stroke
* These authors contributed equally

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Dit protocol beschrijft een confocale beeldvormingstechniek om drie fusiemodi in boviene bijnierchromaffinecellen te detecteren. Deze fusiemodi omvatten 1) close-fusion (ook wel kiss-and-run genoemd), waarbij fusieporiën worden geopend en gesloten, 2) stay-fusion, waarbij fusieporie wordt geopend en de geopende porie wordt gehandhaafd, en 3) krimpfusie, waarbij gesmolten blaasjeskrimp betrokken zijn.

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Neurowetenschappen Nummer 181
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