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Journal / Bioengineering / Isolering, ekspansion og differentiering af…
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JoVE Journal Bioengineering
Isolering, ekspansion og differentiering af mesenkymale stamceller fra den infrapatellære fedtpude i gedekvæleleddet
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Isolering, ekspansion og differentiering af mesenkymale stamceller fra den infrapatellære fedtpude i gedekvæleleddet

Article DOI: 10.3791/63617-v • 12:06 min • August 2nd, 2022
August 2nd, 2022 •
*1,2, *1,2, 1,3, 1,2
1Department of Biological Sciences and Bioengineering, Indian Institute of Technology-Kanpur, 2The Mehta Family Centre for Engineering in Medicine, Indian Institute of Technology-Kanpur, 3Mechanobiology Institute, National University of Singapore
* These authors contributed equally

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Infrapatellar fedtpude mesenkymale stamceller (IFP-MSC'er) kan let isoleres fra knæleddets infrapatellære fedtpude. De formerer sig godt in vitro, danner CFU-F-kolonier og differentierer sig til adipogene, kondrogene og osteogene slægter. Heri gives metoden til isolering, udvidelse og differentiering af IFP-MSC'er fra gedekvælningsled.

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Bioengineering udgave 186 Gede infrapatellar fedtpude (IFP) mesenkymale stamceller (MSC'er) celleisolering cellekultur multilineagepotentiale regenerativ medicin vævsteknik
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