JoVE Journal
Biology
Biology
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
The present protocol describes the isolation of microRNAs from tick salivary glands and purified extracellular vesicles. This is a universal procedure that combines commonly used reagents and supplies. The method also allows the use of a small number of ticks, resulting in quality microRNAs that can be readily sequenced.