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Journal / Developmental Biology / Etablering av organoider fra human tann som et…
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JoVE Journal Developmental Biology
Etablering av organoider fra human tann som et kraftig verktøy mot mekanistisk forskning og regenerativ terapi
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Etablering av organoider fra human tann som et kraftig verktøy mot mekanistisk forskning og regenerativ terapi

Article DOI: 10.3791/63671-v • 11:02 min • April 13th, 2022
April 13th, 2022 •
1, 2, 2, 1
1Laboratory of Tissue Plasticity in Health and Disease, Cluster of Stem Cell and Developmental Biology, Department of Development and Regeneration, Leuven Stem Cell Institute, KU Leuven (University of Leuven), 2Biomedical Research Institute (BIOMED), UHasselt, Hasselt University

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Vi presenterer en protokoll for å utvikle epiteliale organoidkulturer som starter fra menneskelig tann. Organoidene er robust utvidbare og rekapitulerer tannens epiteliale stamceller, inkludert deres ameloblast differensieringskapasitet. Den unike organoidmodellen gir et lovende verktøy for å studere human tann (stamcelle) biologi med perspektiver for tannregenereringsmetoder.

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Utviklingsbiologi utgave 182
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