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Journal / Biochemistry / Evaluación de la función mitocondrial en el…
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Biochemistry
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JoVE Journal Biochemistry
Evaluación de la función mitocondrial en el nervio ciático mediante respirometría de alta resolución
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Journal (Biochemistry)
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Evaluación de la función mitocondrial en el nervio ciático mediante respirometría de alta resolución

Article DOI: 10.3791/63690-v • 08:19 min • May 5th, 2022
May 5th, 2022 •
1, 1
1Instituto de Bioquímica Médica Leopoldo de Meis, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro

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La respirometría de alta resolución acoplada a sensores de fluorescencia determina el consumo de oxígeno mitocondrial y la generación de especies reactivas de oxígeno (ROS). El presente protocolo describe una técnica para evaluar la frecuencia respiratoria mitocondrial y la producción de ROS en el nervio ciático permeabilizado.

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Bioquímica Número 183
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