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Journal / Biochemistry / MALDI-TOF 질량 분광법을 이용한 효소적 분해로 인한 RNA 단편의…
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Biochemistry

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JoVE Journal Biochemistry
MALDI-TOF 질량 분광법을 이용한 효소적 분해로 인한 RNA 단편의 확인
The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes
Journal (Biochemistry)
The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes
Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases
Journal (Biochemistry)
Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases
 
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MALDI-TOF 질량 분광법을 이용한 효소적 분해로 인한 RNA 단편의 확인

Article DOI: 10.3791/63720-v • 09:20 min • April 11th, 2022
April 11th, 2022 •
1, 2, 2, 3, 1
1Department of Chemistry, University of Colorado, Denver, 2Department of Chemistry & Biochemistry, University of Denver, 3Analytical Resources Core Bioanalysis & Omics, Colorado State University

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MALDI-TOF는 산화된 RNA와 엑소리보뉴클레아제 Xrn-1 사이의 반응성으로부터 수득된 단편을 특성화하는데 사용되었다. 본 프로토콜은 RNA 및/또는 DNA를 포함하는 다른 공정에 적용될 수 있는 방법론을 기술한다.

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생화학 문제 182 MALDI-TOF RNA 특성화 핵산 질량 분석법 RNA의 8-oxoG 리보뉴클레아제 반응성 효소 분해
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