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Journal / Cancer Research / Manutenção da Diversidade Celular Glioblastoma…
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JoVE Journal Cancer Research
Manutenção da Diversidade Celular Glioblastoma Humano Ex vivo usando cultura organoide tridimensional
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Manutenção da Diversidade Celular Glioblastoma Humano Ex vivo usando cultura organoide tridimensional

Article DOI: 10.3791/63745-v • 07:11 min • August 25th, 2022
August 25th, 2022 •
1, 2, 1, 2
1Department of Neurological Surgery, Cleveland Clinic, 2Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic

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Aqui, descrevemos um método de geração de organoides de glioblastoma (GBM) a partir de amostras de pacientes primários ou culturas celulares derivadas do paciente e mantê-los até a maturidade. Esses organoides GBM contêm populações de células fenotipicamente diversas e recriam microambientes tumorais ex vivo.

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Pesquisa sobre câncer edição 186
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