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JoVE Journal Biochemistry
En kompetent hepatocyttmodell som undersøker hepatitt B-virusinngang gjennom natriumtaurokolat som transporterer polypeptid som terapeutisk mål
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En kompetent hepatocyttmodell som undersøker hepatitt B-virusinngang gjennom natriumtaurokolat som transporterer polypeptid som terapeutisk mål

Article DOI: 10.3791/63761-v • 11:34 min • May 10th, 2022
May 10th, 2022 •
1, 2, 2, 3,4, 5
1Department of Biochemistry, Faculty of Pharmacy, Mahidol University, 2Program in Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, 3Excellent Center for Drug Discovery, Faculty of Science, Mahidol University, 4Department of Biotechnology, Faculty of Science, Mahidol University, 5Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University

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Vi presenterer en protokoll for å screene anti-hepatitt B-virus (HBV) forbindelser rettet mot pre- og post-viral oppføring livssyklus stadier, ved hjelp av isotermisk titrering kalorimetri for å måle binding affinitet (KD) med vert natrium taurocholate cotransporting polypeptid. Antiviral effekt ble bestemt gjennom undertrykkelse av virale livssyklusmarkører (cccDNA-dannelse, transkripsjon og viral montering).

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Biokjemi utgave 183 Hepatitt B inngangshemmer hepatocytt NTCP HBV ITC bindingsanalyse TCA-opptak isotermisk titreringskalorimetri
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