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JoVE Journal Developmental Biology
Beredning och morfologisk analys av kycklingkraniala neurala vapencellkulturer
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Beredning och morfologisk analys av kycklingkraniala neurala vapencellkulturer

Article DOI: 10.3791/63799-v • 09:26 min • June 27th, 2022
June 27th, 2022 •
1, 2,3, 2,3, 2,3
1Department of Biology and Neuroscience Program, Hamline University, 2Department of Genetics, Cell Biology and Development, University of Minnesota, 3Developmental Biology Center, University of Minnesota

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Detta mångsidiga protokoll beskriver isoleringen av premigrativa neurala vapenceller (NCC) genom excision av kraniala neurala veck från kycklingembryon. Vid plätering och inkubation uppstår migrerande NCC från neurala vikplantor, vilket möjliggör bedömning av cellmorfologi och migration i en förenklad 2D-miljö.

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Utvecklingsbiologi utgåva 184
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