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Journal / Developmental Biology / Isolering av helcellsproteinlysater från musens…
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JoVE Journal Developmental Biology
Isolering av helcellsproteinlysater från musens ansiktsprocesser och odlade palatala mesenkymceller för fosfoproteinanalys
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Isolering av helcellsproteinlysater från musens ansiktsprocesser och odlade palatala mesenkymceller för fosfoproteinanalys

Article DOI: 10.3791/63834-v • 07:26 min • April 1st, 2022
April 1st, 2022 •
*1, *1, 1
1Department of Craniofacial Biology, School of Dental Medicine, University of Colorado Anschutz Medical Campus
* These authors contributed equally

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Protokollet presenterar en metod för att isolera helcellsproteinlysater från dissekerade ansiktsprocesser för musembryon eller odlade musembryonala palatala mesenkymceller och utföra efterföljande western blotting för att bedöma fosforylerade proteinnivåer.

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Utvecklingsbiologi utgåva 182 Kraniofacial utveckling fosforylering fosfoproteiner mus maxillära processer musembryonala palatala mesenkymceller
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